General Cell Based Assays
Molecular Devices Cell Integrity Kit, Fluorescent, Live/Dead, 96- and 384-Well
The Molecular Devices EarlyTox Cell Integrity Kit R8214 is a fluorescence-based assay for rapid differentiation of live and dead cells using complementary nuclear dyes. The bulk kit is formulated for high-throughput imaging in 96- and 384-well plates and supports a homogeneous mix-and-read workflow with an optional masking reagent for improved signal.
- Sufficient for ten 96-well plates (approximately 960 wells) or equivalent 384-well plates.
- Contains two nuclear dyes for live and dead cell discrimination and masking reagent for homogeneous assays.
- Assay principle based on membrane permeability detection for live/dead identification.
- Compatible with adherent and non-adherent cell types and high-throughput imaging workflows.
- Recommended plate types: black-well, clear-bottom plates for imaging.
Eurofins Discoverx PathHunter eXpress GALR1 Total Internalization Assay, 10pl Kit. (93-0781E3CP5L)
The PathHunter GALR1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GALR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GALR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GALR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology Phospho-Lamin A/C-S22 Rabbit pAb
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Cayman Chemical THUNDRPhosphop38a MAPK 9600Wel
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates
Eurofins Discoverx PathHunter eXpress GPR88 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0357E2ACP1M)
The PathHunter GPR88 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR88 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR88 and EA-tagged β-Arrestin-2. Activation of GPR88 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences Rap1 Assay Reagent Ral GDS
Product Source expressed in E. coli
Eurofins Discoverx PathHunter eXpress EDG7 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0636E2CP13M)
The PathHunter EDG7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG7 and EA-tagged β-Arrestin-2. Activation of EDG7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 32 Reagent, 500. (93-0563R32B)
AssayComplete Cell Plating 32 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical AMPMaxSpec KIt 15test
Cayman’s AMP+ MaxSpec Kit contains a positively charged reagent (AMP+) that can be coupled quantitatively to free carboxylic acids through an amide bond linkage. The resulting derivatized product thus contains a positively charged group that shows improved electrospray ionization and consequently improved MS sensitivity. The derivatization reagent is stable to MS/MS fragmentation and thus isobaric derivatives undergo compound-specific fragmentation to give unique fragment ions.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 10,000 Assay Points
Abcam Ethanol Assay Kit
Ethanol Assay Kit
The product is subject to the following: Abcam Restricted Use Statement
Meso Scale Discovery U-PLEX Human ICAM-1 ASY
The Meso Scale Discovery U-PLEX Human ICAM-1 Assay Kit is designed to quantitatively measure Intercellular Adhesion Molecule 1 (ICAM-1) in human serum plasma and cell culture supernatants using an electrochemiluminescent detection method This assay kit includes a 96-well plate coated with capture antibody detection antibody conjugated to an electrochemiluminescent label calibrator and buffer for sample dilution The assay has a detection range of 78-50 000 pg/mL and exhibits no cross-reactivity with other adhesion molecules This product can aid in understanding ICAM-1 levels related to inflammatory and immune responses
Meso Scale Discovery R-PLEX Mouse M-CSF ASY
The R-PLEX Mouse M-CSF Assay from Meso Scale Diagnostics is a multiplex immunoassay kit for measuring mouse macrophage colony stimulating factor (M-CSF) in various biological fluids This ECL-based assay uses MULTI-ARRAY technology and includes a pre-coated 96-well plate calibrators controls diluent detection antibody read buffer and seals It has a sensitivity of 0 3 pg/mL and an upper limit of 10 000 pg/mL The assay offers high sensitivity low sample volume and quick results
Cayman Chemical GGT Substrate DIluent 3.5mL
GGT substrate diluent has been tested and formulated to work exclusively with Cayman’s γ-Glutamyl Transferase Activity Assay Kit (Item No. 702210).
Eurofins Discoverx cAMP Hunter eXpress ADRB2 (B2AR) CHO-K1 GPCR Assay, 10-Plate Kit. (95-0121E2CP2L)
The cAMP Hunter ADRB2 (B2AR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB2, a naturally Gs coupled GPCR. Upon activation of ADRB2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.